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. 2020 Jul 31;10:12922. doi: 10.1038/s41598-020-69472-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MS diet protects mice from AIEC intestinal colonization and inflammation. (a) Experimental protocol of infection used in the study. (b–d) AIEC LF82 load in (b) the stools at days 1, 2 and 3 post-infection, (c) associated to ileum and (d) associated to colonic mucosa was quantified on selective medium (CTR group: n = 11, MS diet: n = 13–15, two independent experiments pooled). (e) Micro-inflammation marker Lcn-2 was quantified in the stools, at days 1, 2 and 3 post-infection by ELISA (CTR group: n = 5, MS diet: n = 5). (f–g) Pro-inflammatory cytokines KC (Keratinocyte Chemoattractant) (f) and IL-6 (g) were quantified in colonic mucosa release medium by ELISA (n = 5 for each group). Mann–Whitney test. *p < 0.05; **p < 0.01; ***p < 0.001.