Fig. 6.

Decreased expression of the gonadotropin-releasing hormone (GnRH) receptor impaired GnRH-mediated accelerated NET generation (NETosis) in the HL-60 cell line. a Suppression of GnRH receptor expression by GnRH receptor-specific siRNAs inhibited GnRH receptor expression in the HL-60 cell line. b GnRH treatment did not increase the expression of citrullinated histone H3 in the GnRH receptor siRNA-transfected HL-60 cell line. c Real-time polymerase chain reaction analysis showed that the GnRH receptor siRNA-transfected HL-60 cell line did not have increased PAD4 expression. d The NETosis assay. GnRH agonist treatment did not promote GnRH-mediated increased NETosis in GnRH receptor siRNA-transfected HL-60 cells. A representative example of four independent experiments is shown. ^*P < 0.05, ^**P < 0.005 and ^***P < 0.0005