Table 2.
Reagents that induce ACOD1 expression
| Compound | Target | Host cell | Induction time | Concentration | Fold change | Function | Animal Model | Ref |
|---|---|---|---|---|---|---|---|---|
| Infection | ||||||||
| LPS | TLR4 | RAW264.7 cells |
4–6 h 6–9 h 6 h |
50 ng/ml 10 ng/ml (055:B5) 10 ng/ml or 5 µg/ml |
25 >400 NA |
NA |
NA NA NA |
8, 50, 40, 53 |
| ANA-1 cells | 16 h | 100 ng/ml (055:B5) | 50 | NA | NA | 14 | ||
| J774A.1 cells | 6 h | 5 µg/ml | NA | NA | NA | 40 | ||
| BMDMs |
6 h 6 h 16 h 24 h |
5 µg/ml 10 ng/ml 100 ng/ml (055:B5) 100 ng/ml |
NA NA 500 >50 |
NA Promote itaconate production NA Promote itaconate production |
NA NA NA NA |
40, 7, 14, 30 | ||
| A20−/− BMDMs |
1 h 6 h |
10 µg/ml (0111:B4) |
3 1.44 |
NA | NA | 53 | ||
| WT, Myd88−/−, Ifnar−/−, and Stat−/− murine DCs | 4 h | 100 ng/ml (055:B5) | NA | NA | NA | 10 | ||
| Tlr4-overexpressing ovine blood macrophages | 1 h | 1 µg/ml | 3 | Anti-inflammatory activity | NA | 45 | ||
| Human monocyte-derived macrophages | 3 h | 100 ng/ml (0111:B4) | 125 | Promote itaconate production | LPS-infected C57BL/6 mice | 41 | ||
| Human PBMC-derived monocytes, macrophages, and DCs | 6–9 h | 10 ng/ml (055:B5) | >400 | NA | NA | 50 | ||
| Human PBMC-derived monocytes | 4 h | 10 ng/ml (055:B5) | 25 | NA | NA | 15 | ||
| Lipid A | TLR4 | WT and Myd88−/− PMs |
4 h 8 h |
1 µg/ml | NA | NA | NA | 44 |
| IFNB | IFNAR |
ANA-1 cells BMDMs |
16 h 16 h 24 h |
10 ng/ml 10 ng/ml 1000 U/ml |
9 3 >4 |
NA NA Promote itaconate production |
NA | 14, 30 |
| IFNG | IFNGR | ANA-1 cells | 16 h | 10 ng/ml | 33.91 | NA | NA | 14 |
| Human PBMC-derived monocytes, macrophages, and DCs | 6–9 h | 10 ng/ml | >400 | NA | NA | 50 | ||
| TNF | TNFR | ANA-1 cells | 16 h | 10 ng/ml | 5.89 | NA | NA | 14 |
| Human PBMC-derived monocytes, macrophages, and DCs | 6–9 h | 50 ng/ml | >400 | NA | NA | 50 | ||
| A20−/− BMDMs |
1 h 6 h |
1000 U/ml |
2.45 2.27 |
NA | NA | 53 | ||
| LTA | TLR2 |
ANA-1 cells BMDMs |
16 h | 1 µg/ml |
9 50 |
NA | NA | 14 |
| BoNT/A | TLR2 | RAW264.7 cells |
4 h 2 h |
1 nM 5 nM |
28.04 319.27 |
NA | NA | 29 |
| Poly I:C | TLR3 | BMDMs | 24 h | 40 µg/ml | >50 | NA | WT and Ifnar1−/− C57BL/6 mice | 30 |
| CpG1668 DNA | TLR9 | ANA-1 cells | 16 h | 1 µM | 60 | NA | NA | 14 |
| CpG DNA | TLR9 | WT, Ifnar−/−, and Stat−/− murine DCs | 4 h | 0.1 µM | NA | NA | NA | 10 |
| KMRC011 | TLR5 | Mouse small intestine | Day 5 post TBI | 0.2 mg/kg | >2 | NA | Total-body irradiation (TBI) C57BL6/N mice | |
| IL1B | IL1R |
ANA-1 BMDMs |
16 h | 10 ng/ml |
10 3 |
NA | NA | 14 |
| Pregnancy | ||||||||
| Pregnancy | Uteri | Day 4 pregnancy | NA | > 75 | Facilitate embryo implantation | CD1 female mice | 13 | |
| Progesterone | PR | Uteri | Day 4 pregnancy |
40 mg/kg 1 mg Additional 50 ng estradiol |
>100 >50 >75 |
Facilitate embryo implantation | Ovariectomized CD1 female mice | 13, 18 |
| LIF | Uteri | 12 h |
5 µg 50 ng/ml |
3.7 40 |
Facilitate embryo implantation | WT and Lif−/− MF1 female mice | 33, 34 | |
| Other | ||||||||
| CHX | RAW264.7 cells | 4 h | 50 µM | NA | NA | NA | 8 | |
| CoPP | RAW264.7 cells | 8–16 h | 10 µM | 8 |
Regulate A20 expression Anti-inflammatory activity |
LPS-infected C57BL6 mice | 38 | |
| Hemin | RAW264.7 cells | 8–16 h | 10 µM | 6.5 |
Regulate A20 expression Anti-inflammatory activity |
LPS-infected C57BL6 mice | 38 | |
| CORM-2 | RAW264.7 cells | 8–16 h | 20 µM | 4.5 |
Regulate A20 expression Anti-inflammatory activity |
LPS-infected C57BL6 mice | 38 | |
| Blast overpressure | BV-2 cells | Pulse duration of 12 ms | 3.7 | NA | NA | 39 | ||
| MSU | Air pouch membrane | 9 h | 2 mg/ml | 19.8 | NA | BALB/c mice | 37 | |