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. 2020 Aug 1;7:129. doi: 10.1038/s41438-020-00351-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Amino acid sequence alignment of CmBES1 with BES1 sequences from various plant species. The single red lines indicate the conserved BES1-N region in BES1-like proteins. The black color indicates 100% identity; red, 75% identity; and blue, 50% identity. The sequences compared to CmBES1 were Arabidopsis thaliana AtBES1 (AT1G19350), AtBZR1 (AT1G75080), AtBEH1 (AT3G50750.1), AtBEH2 (AT4G36780), AtBEH3 (AT4G18890), and AtBEH4 (AT1G78700); Helianthus annuus BES1/BZR1 homolog protein 2-like (HaBEH2-like, LOC110908961); Cynara cardunculus BES1/BZR1 homolog protein 2-like (CcBEH2-like, LOC112512677); and Populus euphratica BES1/BZR1 homolog protein 2-like (PeBEH2-like, LOC105107647). b Phylogenetic tree comprising the following CmBES1 and BES1 family proteins: Arabidopsis thaliana AtBES1 (AT1G19350), AtBZR1 (AT1G75080), AtBEH1 (AT3G50750.1), AtBEH2 (AT4G36780), AtBEH3 (AT4G18890), and AtBEH4 (AT1G78700); Arachis ipaensis AiBEH2 (XP_016201823.1); Artemisia annua AaBES1/BZR1-like (PWA37330.1); Cucumis sativus CsBEH2 (XP_004143497.1); Cynara cardunculus CcBEH2-like (LOC112512677); Eucalyptus grandis EgBEH2-like (NP_001306904.1); Helianthus annuus HaBEH2-like (LOC110908961); Hevea brasiliensis HbBEH2-like (XP_021646974.1); Ipomoea triloba ItBEH2-like (XP_031095547.1); Jatropha curcas JcBEH2 (XP_012079081.1); Lactuca sativa LsBEH2-like (XP_023759843.1); Malus domestica MdBEH2-like (XP_008338783.2); Manihot esculenta MeBEH2 (XP_021632984.1); Momordica charantia McBEH2 (XP_022132946.1); Morus notabilis MnBEH2 (XP_024020611.1); Populus euphratica PeBEH2-like (LOC105107647); Populus trichocarpa PtBEH2 (XP_002310201.1); Prunus avium PaBEH2-like (XP_021809451.1); Quercus lobata QlBEH2-like (XP_030949816.1); Ricinus communis RcBEH2 (XP_002525100.1); Sesamum indicum SiBEH2-like (XP_011097209.1); Spinacia oleracea SoBEH2 (XP_021835401.1); Tanacetum cinerariifolium TcBEH2-like (GEY43666.1); and Ziziphus jujuba ZjBEH2 (XP_015890203.1). The phylogenetic tree was constructed using the neighbor-joining method and bootstrap test with 1000 replicates. The divergence of each branch is indicated by the bootstrap values