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. 2020 Aug 1;7:129. doi: 10.1038/s41438-020-00351-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Transcriptional activity of CmBES1 measured in a yeast assay system. Y2H cells expressing pCL1 grew on SD/-His-Ade media and served as positive controls. Y2H cells expressing pGBKT7 could not grow on this media and served as negative controls. SD/-T/-L: SD/-Trp media (pGBKT7 and genes) or SD/-Leu media (pCL1), SD/-HA: SD/-His/-Ade media, SD/-HA + X-α-gal: SD/-His/-Ade media+X-α-gal. b Relative luciferase activities in Arabidopsis mesophyll protoplasts after transfection with 35 S::GAL4DB-CmBES1; the 35 S::GAL4DB-AtARF5 construct served as a positive control, and 35 S::GAL4DB served as a negative control