Table 1.
CM3G1 | CM3G2 | CM4G3 | CM4G4 | |
---|---|---|---|---|
Regenerated lines | 117 | 63 | 22 | 8 |
Cas9 positive lines | 68 | 39 | 13 | 5 |
Homozygousa lines | 0 | 1 | 0 | 0 |
Heterozygousb lines | 1 | 3 | 3 | 0 |
Biallelicc lines | 2 | 0 | 1 | 0 |
Chimericd lines | 7 | 1 | 1 | 0 |
Nonedited lines | 107 | 58 | 17 | 8 |
Editing efficiency | 14.7% | 12.8% | 38.5% | 0 |
aHomozygous = both alleles contain the same mutation.
bHeterozygous = only one allele is mutated.
cBiallelic = both alleles are mutated, but the mutations are not identical.
dChimeric = presence of more than two mutant alleles, indicative of the presence of different edited cell lines.