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. 2020 Jul 31;10:12902. doi: 10.1038/s41598-020-69499-6

Figure 6.

Figure 6

Roniciclib interferes with cancer stem cell markers and induces nucleolar fragmentation in neuroblastoma cells. Immunofluorescence analysis of IMR-32, ACN and SH-SY5Y neuroblastoma cell lines untreated (Roniciclib 0 µM) or treated with Roniciclib 1 µM, 20 µM and 5 µM respectively for 72 h, using anti-CD44v6, anti-CD114, anti-NCL, anti-NPM1, anti-GPC2 and anti-PES1 (all green) antibodies. White arrows indicate nucleolar fragmentation with nucleoplasmic/cytoplasmic redistribution of NPM1 and PES1 proteins. Cells were counterstained with DAPI to visualize nuclei (blue). (Scale bars: 20 µm).