Table 1.
Reference | Product size (bp) | Ta | Nucleotide sequence 5’ → 3’ |
Primer |
---|---|---|---|---|
( 18 ) | 293 | 60C | 5′-ACCAAG CTGAGCGA GTGTC-3′ | BAX (F) |
( 18 ) | 293 | 60C | 5′-ACAAAGATGGTCACGGTCTGCC-3′ | BAX (R) |
( 18 ) | 415 | 60C | 5′-ACCAAG CTGAGCGA GTGTC-3′ | Bcl-2 (F) |
( 18 ) | 415 | 60C | 5′-ACAAAGATGGTCACGGTCTGCC-3′ | Bcl-2 (R) |
( 18 ) | 473 | 64C | 5′-GTTTCCGTCTGGGCTTCTTG-3′ | P53 (F) |
( 18 ) | 473 | 64C | 5′-CCTGGGCATCCTTGAGTTCC-3′ | P53 (R) |
( 18 ) | 517 | 60C | 5′-CTCAGAGGAGGCGCCATG-3′ | P21 (F) |
( 18 ) | 517 | 60C | 5′-GGGCGGATTAGGGCTTCC-3′ | P21 (R) |
( 18 ) | 350 | 60C | 5′-AACCGCGAGAAGATGACCCAGATCATGTTT-3′ | β-actin (F) |
( 18 ) | 350 | 60C | 5′-AGCAGCCGTGGCCATCTCTTGCTCGAAGTC-3′ | β-actin (R) |
F: Forward; R: Reverse; bp: Base pair