Fig. 3.

Calorimetric thermal unfolding of SARS-CoV-2 3CLpro. (a) Molar excess heat capacity as a function of temperature at pH 5 (black), pH 7 (red), and pH 8 (green), at 14 μM protein concentration. (b) Molar excess heat capacity as a function of temperature at pH 8. The experimental data (circles) and the non-linear regression analysis fitting curves (continuous lines) according to two-state monomeric (gray), dimeric (green), tetrameric (red), and octameric (blue) protein unfolding models are shown.