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. 2020 Aug 1;164:1693–1703. doi: 10.1016/j.ijbiomac.2020.07.235

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Fig. 4 — Catalytic activity of SARS-CoV-2 3CLpro. Time evolution of the substrate fluorescence emission as a function of time at pH 5 (black), pH 7 (red), and pH 8 (green), at protein concentration of 1 μM and substrate concentration of 20 μM. The increase in fluorescence emission reflects the decrease in Dabcyl quenching on Edans emission (i.e., reduction of FRET phenomenon) due to the increase in the spatial separation of the donor-acceptor pair as a result of the catalytic cleavage of the substrate molecule.