Table 1.
Different RT-qPCR solutions evaluated for the detection of SARS-CoV-2 in nasopharyngeal swab samples from COVID-19 positive patients.
| Solution | #Targetsa | Target gene | Sensitivity, % (95% CI)b | FNc |
|---|---|---|---|---|
| TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) combined with validated primer-probe setsd | 3 | E | 95.9 (89.9–98.9) | 4 |
| N | 75.5 (65.8–83.6) | 24 | ||
| RdRp | 77.6 (68.0–85.4) | 22 | ||
| LightMix® Modular SARS-CoV (COVID19) (TIB MOLBIOL) | 3 | E | 97.9 (92.8–99.7) | 2 |
| N | 78.6 (69.1–86.2) | 21 | ||
| RdRp | 89.8 (82.0–95.0) | 10 | ||
| SARS-COV-2 R-GENE (BioMérieux) | 3 | E | 65.3 (55.0–74.6) | 34 |
| N | 66.3 (56.1–75.6) | 33 | ||
| RdRp | 60.2 (49.8–70.0) | 39 | ||
| TaqPath COVID-19 CE-IVD RT-PCR Kit (Thermo Fisher Scientific) | 3 | ORF1ab | 65.3 (55.0–74.6) | 34 |
| S | 70.4 (60.3–79.2) | 29 | ||
| N | 76.5 (66.9–84.5) | 23 | ||
| Genesig Real-Time PCR COVID-19 kit (Primedesign Ltd.) | 1 | RdRp | 81.6 (72.5–88.7) | 18 |
| Real Accurate Quadruplex corona-plus PCR Kit (PathoFinder) | 1 | N | 83.7 (74.8–90.4) | 16 |
a
Specific primer-probes for SARS-CoV-2.
b
95% Confidence Interval.
c
False negative counts out of 98 patients.