Figure 2.

I-MDSCs are reduced in Apc^Min/+ mice treated with GSK 872. A, Representative flow cytometry plots of WT monocytes and neutrophils, and Apc^Min/+ MDSC subsets; percentages of M-MDSCs (B), I-MDSCs (C), and PMN-MDSCs (D) gated on CD45⁺CD11b^hiCD11c^lo live splenocytes, macrophages (CD11b⁺F4/80⁺; E and F), and CD45⁺ (I) cells from Apc^Min/+ mice receiving vehicle control (n = 4) or GSK 872 (n = 5). G and H, Apc^Min/+ MDSC subsets suppress CFSE-labeled, polyclonally activated (anti-CD3 and anti-CD28 antibodies) WT, CD4, and CD8 T-cell proliferation. Representative flow cytometry plots show the suppression of CD4 and CD8 T cells by MDSCs (1:1 MDSCs to T cells). Activated T cells without MDSC coculture were positive controls. Comparisons were made between positive control and each MDSC to T-cell coculture. *, P < 0.05; ns, nonsignificant.