Figure 5.

Growth of 3D cellular structures printed with magnetic assistance and formed under the influence of gravity alone. (i) Maximum projected area and (ii) circularity measurements of 3D cellular structures (a) printed with magnetic assistance and (b) formed through gravitational settling. The maximum projected areas of the monotypic MDA-MB-231 and 12.5 and 25% fibroblast coculture 3D structures printed with magnetic assistance decrease by 39, 64, and 55%, respectively, after their initial formation. The maximum projected areas of 3D structures containing a 50% fibroblast coculture and a monotypic fibroblast decrease by 51 and 57%, respectively, until 24 hours. These maximum projected areas are maintained until 72 hours. From 144 hours until 336 hours, the maximum projected areas increase slightly within experimental error. In contrast, the maximum projected areas of 3D cellular structures formed under the influence of gravity alone ((b), i) increase by 103, 130, 117, and 94% from 144 to 336 hours for monotypic MDA-MB-231 and 12.5, 25, and 50% fibroblast 3D structures, respectively. As the proportion of fibroblasts increases, structure circularity ((a), ii, and (b), ii) also improves. The legend in ((b), i), applies to graphs in panels ((a), i; (a), ii; and (b), ii).