Table 1.
Primer sequence for RT-qPCR.
| Target Gene | Direction | Sequence (5′−3′) |
|---|---|---|
| IL-1β | Forward | GCACGATGCACCTGTACGAT |
| Reverse | AGACATCACCAAGCTTTTTTGCT | |
| Probe | FAM-ACTGAACTGCACGCTCCGGGACTC-TAM | |
| TNF-α | Forward | AACATCCAACCTTCCCAAACG |
| Reverse | GACCCTAAGCCCCCAATTCTC | |
| Probe | FAM-CCCCCTCCTTCAGACACCCTCAACC-TAM | |
| IL-6 | Forward | CGGGAACGAAAGAGAAGCTCTA |
| Reverse | CGCTTGTGGAGAAGGAGTTCA | |
| Probe | FAM-TCCCCTCCAGGAGCCCAGCT-TAM | |
| MCP-1 | Forward | CAAGCAGAAGTGGGTTCAGGAT |
| Reverse | AGTGAGTGTTCAAGTCTTCGGAGTT | |
| Probe | FAM-CATGGACCACCTGGACAAGCAAACC-TAM | |
| IP-10 | Forward | GAAATTATTCCTGCAAGCCAATTT |
| Reverse | TCACCCTTCTTTTTCAT-TGTAGCA | |
| Probe | FAM-TCCACGTGTTGAGATCA-TAM | |
| GAPDH | Forward | GAAGGTGAAGGTCGGAGTC |
| Reverse Probe | GAAGATGGTGATGGGATTTC FAM-CAAGCTTCCCGTTCTCAGCC-TAM |