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. 2019 Oct 10;105(8):2071–2082. doi: 10.3324/haematol.2019.224899

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Figure 1. — Tfr1 expression in hematopoietic stem/progenitor cells. (A) Representative gating strategy for analyzing long-term hematopoietic stem cells (HSC) (LT-HSC, Lin⁻cKit⁺Sca1^hiCD34⁻Flt3⁻), short-term HSC (ST-HSC, Lin⁻cKit⁺Sca1^hiCD34⁺Flt3⁻), multipotent progenitor (MPP, Lin⁻cKit⁺Sca1^hiCD34⁺ Flt3⁺) cells, common myeloid progenitor (CMP, Lin⁻cKit⁺Sca1-CD34⁺ CD16/32^int) cells, granulocyte-macrophage progenitor (GMP, Lin-cKit⁺Sca1-CD34⁺CD16/32^hi) cells, megakaryocyte-erythroid progenitor (MEP, Lin⁻cKit⁺Sca1-CD34⁻CD16/32^lo) cells, and common lymphoid progenitor (CLP, Lin⁻cKit^intSca1^intIL-7R⁺Flt3⁺) cells. (B) Erythrocytes gating strategy was used based on the expression levels of Ter119 and CD44: R1, proerythroblasts (Ter119^loCD44^hi); R2, early basophilic erythroblasts (CD44^hiFSC^hi); R3, polychromatophilic erythroblasts (CD44^hiFSC^med); R4, orthochromatophilic erythroblasts (CD44^medFSC^med); and R5, mature erythrocytes (CD44^loFSC^lo). (C) Flow cytometric analysis of Tfr1 expression in HSPC and erythroblasts in fetal liver of E16.5 mouse embryos. Grey histogram: isotype control (Rat IgG2a, k-PE). (D) Quantification of the flow cytometry data in C, showing the mean fluorescence intensity (MFI) of Tfr1 in the indicated hematopoietic stem/progenitor cells (HSPC) and erythroblasts.