Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 10;105(8):2150–2163. doi: 10.3324/haematol.2019.220525

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright© 2020 Ferrata Storti Foundation

Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions:

https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions:

https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher.

PMC Copyright notice

Figure 3. — Priming correlates with sensitivity to BH3-mimetics. The interaction of anti- and pro-apoptotic BCL-2 proteins was investigated in a selection of ten cell lines with varying sensitivities to BH3-mimetics. Immunoprecipitation of BCL-2, BCL-X_L and MCL-1 was performed in untreated cell lysates followed by analysis of binding of pro-apoptotic BCL-2 proteins (BIM, NOXA, BAX and BAK) using Western blotting. Protein G beads without primary antibody were used to control for unspecific binding. Staining with BCL-2, BCL-X_L, MCL-1 and GAPDH was performed to demonstrate efficient immunoprecipitation and equal protein loading, respectively. Representative western blots of two independent experiments are shown.