Figure 2.

Localization of reporter-proteins by immunofluorescence confocal imaging. The reporter-protein factor IX gla-protein C (FIXgla-PC) with the propeptide of FIX or bone Gla protein (BGP) was transiently expressed in COS-7 cells. The transfected cells were cultured with complete medium containing 11 μM vitamin K. The carboxylated reporter-protein (carboxylated PC) was immuno-stained by the mouse anti-carboxylated FIXgla monoclonal antibody as the primary antibody, and Alexa Fluor-488 conjugated donkey anti-mouse IgG as the secondary antibody (green image). The total reporter-protein (PC) was probed by the sheep anti-PC polyclonal antibody as the primary antibody and the Alexa Fluor-568 conjugated donkey anti-sheep IgG as the secondary antibody (red image). The cell nucleus was stained by Hoechst 33342 (blue image).