Figure 1.

LJ000328 is selective of the BMP Type I receptors and is a potent inhibitor of liver hepcidin expression. (A) LJ000328 chemical structure. (B) Selectivity of LJ000328 for different BMP, TGF-β, and activin receptors, determined by in vitro kinase assays. Wild-type (WT) C57BL/6 male mice injected with LJ000328 (blue boxes) (8-10/group) were sacrificed 2, 6, and 15 hours after injection and compared to mock-injected mice (grey boxes). Mice were analyzed for liver (C) P-SMAD5 relative to total SMAD5 protein expression and (D) Hamp mRNA expression. Box-and-whisker plots are shown for Hamp ΔCt values. Mean ΔCt values were compared by a Student’s t-test. ****P<0.0001.