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. 2019 Nov 28;105(8):2044–2055. doi: 10.3324/haematol.2019.226936

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Figure 6. — Low doses (LD) of ionizing radiations (IR) induce a transitory increase of ROS in CD34⁺ CD38^low CD45RA⁻CD90⁺ hematopoietic stem progenitor cells (HSPC) that alters their serial clonogenic potential. (A) Colony forming unit-cell (CFU-C) assay. Cumulative results from 3 independent experiments with CD34⁺ CD38^low CD45RA⁻CD90⁺ HSPC from 3 independent pools of cord blood (CB) samples. Sorted CD34⁺ CD38^low CD45RA⁻ CD90⁺ HSPC were pre-treated or not with N-acetylcysteine (NAC) prior to IR and plated (500 cells/plate) in CFU-C conditions for 12-14 days. Shown are the number (nb) of CFU-C (primary CFU-C). Results are normalized to the sham-irradiated conditions. (B) Primary CFU-C were pooled and replated in CFU-C conditions for 12-14 days. Shown are the nb of secondary CFU-C, normalized to the sham-irradiated conditions (cumulative results from 3 independent experiments). (C) Sorted CD34⁺ CD38^low CD45RA⁻ CD90⁺ HSPC were pre-treated or not with SB203580 prior to IR and plated (500 cells/plate) in CFU-C conditions for 12-14 days. Shown are the nb of CFU-C (primary CFU-C). Results are normalized to the sham-irradiated conditions. (D) Primary CFU-C were pooled and replated in CFU-C conditions for 12-14 days. Shown are the nb of secondary CFU-C, normalized to the sham-irradiated conditions (cumulative results from 2 experiments with CD34⁺ CD38^low CD45RA⁻ CD90⁺ HSPC from two independent pools of CB samples. (E) Model explaining how LDIR can impair HSC self-renewal through ROS-p38MAPK dependent pathway. Results are shown as mean+standard error of mean. ^**P<0.01, ^***P<0.001, ^****P<0.0001 (Mann-Whitney statistics).