Fig. 2.
SERCA overexpression improves endothelial function under H/R conditions. Primary CMECs were cultured in a vascular-cell basal medium supplemented with the endothelial cell growth kit VEGF. Hypoxia/reoxygenation (H/R) injury was induced through 30 min of hypoxia and 2 h of reoxygenation. SERCA AAV9 or control AAV9 vectors were transfected into CMECs, which were termed SERCAAAV9 group or control group respectively. A–C. Proteins were isolated from H/R-treated CMECs, and then the levels of phosphorylated eNOS and ET-1 were measured through western blots. D–E. RNA was isolated from H/R-treated CMECs, and the transcriptions of ICAM1 and VCAM1 were determined through qPCR. F–G. Endothelial barrier function was determined through the FITC-dextran experiment and TER assay. *p < .05.