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. 2019 Dec 25;123(2):670–681. doi: 10.1152/jn.00500.2019

Fig. 6.

Fig. 6.

High-amplitude inhibitory postsynaptic currents (IPSCs) are less sensitive to 5-HT than low-amplitude IPSCs. Recordings were performed in the presence of CNQX (10 µM), dl-APV (50 µM), and CGP55845 (1 µM). A: representative traces showing IPSCs in control conditions (black) and in the presence of 5-HT agonists (5- HT; 5 µM, n = 20; CP93129; 5 µM; n = 8; red) in cells with high- (top) and low-amplitude (bottom) IPSCs. The low-amplitude IPSC was suppressed to a greater extent by the 5-HT agonists than the high-amplitude IPSC. B: IPSC suppression ratio (y-axis) as a function of control IPSC amplitude (x-axis). C: differing time course of low (blue)- and high-amplitude (red) IPSCs. Low amplitude IPSC was scaled up to match the peak amplitude of a high-amplitude IPSC, highlighting the slower decay time of low-amplitude IPSCs. D: IPSC half-width (y-axis) as a function of IPSC amplitude (x-axis). E: for high (left)- and low-amplitude (right) IPSCs, 3 superimposed traces are shown: control IPSC (black), IPSC in the presence of 5-HT agonist (red), and 5-HT-sensitive component (blue). The 5-HT-sensitive IPSC was estimated by subtracting the 5-HT-depressed IPSC from the initial IPSC. F: for high (left)- and low-amplitude (right) IPSCs, we show 3 superimposed traces scaled so that their peak amplitude would match: control IPSC (black), IPSC in the presence of the 5-HT agonist (red) and 5-HT-sensitive component (blue). G: averaged IPSCs recorded in the experiments testing the effects of 5-HT (left) or CP93129 (right), irrespective of control IPSC amplitudes. In both cases, we show 3 superimposed traces scaled so that their peak amplitude would match: control IPSC (black), IPSC in the presence of 5-HT agonist (red), and the difference between these 2 traces.