Fig. 3.

α-Endothelial epithelial Na⁺ channel (αEnNaC) mediates Western diet (WD)-induced and aortic and renal oxidant stress and reduction of endothelial nitric oxide synthase (eNOS) activation. A−C: representative images of immunostaining for aorta 3-nitrotyrosine (3-NT; A), kidney 3-NT (B), and kidney 4-hydroxynonenal (4-HNE) (C) with corresponding quantitative analysis. Scale bars = 50 µm. D: Western blots of Ser¹¹⁷⁷ phosphorylated (p-)eNOS or total eNOS normalized to GAPDH and ratio of p-eNOS to eNOS with representative quantitative analyses. Data are expressed as means ± SD; n = 5 or 6. *P < 0.05 vs. control diet (CD)-fed αEnNaC^+/+ mice; †P < 0.05 vs. WD-fed αEnNaC^+/+ mice.