Fig. 2.
ADCC reporter assay of antisera from cHA-vaccinated mice against target cells expressing the HA of H1N1, H3N2, or H5N1 and subtypes. The antisera collected from mice immunized with cHAfg or cHAmg proteins adjuvanted with aluminum hydroxide or C34 were incubated with MDCK cells which were infected with (A) H1N1 virus (B) H5N1 virus, or (C) H3N2 virus for 30 min. Subsequently, the ADCC reporter assay was performed using Jurkat effector cells expressing mouse FcγRIII, and the relative luminescence unit (RLU) was measured and values are mean ± SEM. ***P < 0.001. The P value was calculated with Prism software using two-way ANOVAs.