Fig. 2.

CCN3 is not required for myelination ex vivo. (A) CCN3 protein quantification via ELISA in cerebellar/brainstem slice conditioned media. Data are mean ± SD. Representative confocal projection images of MBP and NFH staining in brain slices at 7 (B) and 14 days ex vivo (dev) (D). (Scale bars: 25 μm.) Myelination index quantification (MBP⁺NFH⁺ colocalization area/NFH⁺ total area) of brain slices at 7 (C) and 14 dev (E). Data are mean ± SEM. ns, not significant. Statistical analysis: Mann–Whitney U test (C) or unpaired, two-tailed, Student’s t test (E). n = 3 wells containing 3 slices each (A) and 4–5 animals per group (C and E).