Fig. 3.

Myo-sex knockout results in flightless males. (A) Three sgRNAs, starting at positions 253, 290, and 304, were used to target the myo-sex coding region, which spans positions 124 to 5,946. (B) CRISPR/Cas9-mediated myo-sex knockout produced flightless males in both the G₀ and G₁ generations in three independent experiments. G₁ progeny were produced only from crosses between wild-type virgin females and sgRNA+Cas9-injected flying G₀ males. (C) Sequence analysis of mutations in CRISPR/Cas9-edited flightless G₁ progeny. Top line denotes the wild-type sequence; subsequent lines show various mutant sequences. The number of deleted (−) and inserted (+) bases and their occurrence are indicated to the right; in-frame mutations are indicated by an asterisk; deleted bases are denoted by dashes. The PAM sites for the three sgRNAs are highlighted in red.