Table 2.

Proportion of transgenic progeny

Replicate	+	−	Percent+, %
N1 replicate
1	72	372	16.2
2	104	394	20.9
3	84	439	16.1
4	66	362	15.4
5	170	268	38.8
6	107	364	22.7
Total	603	2,199	21.5
N2 replicate
1	57	279	17.0
2	129	378	25.4
3	154	329	31.9
4	148	214	40.9
5	108	118	47.8
6	125	264	32.1
Total	721	1,582	31.3

Generalized linear mixed-effect model analyses were performed to test the null hypothesis that 25% of the progeny will be transgenic under the assumption of equal reproductive fitness between the hemizygous transgenic and nontransgenic males. No statistically significant departure from the 25% expectation was detected for either N1 or N2 (P > 0.05). Power analysis by 1,000 simulations show that the probability to correctly reject the null hypothesis (at α = 0.05) is 98.50% (95% confidence interval: 97.54 to 99.16%) and 99.10% (95% confidence interval: 98.30 to 99.59%) for N1 and N2, respectively. Data for each individual brood are provided in SI Appendix, Table S9. +, Number of transgenic (positive) progeny; −, number of nontransgenic (negative) progeny.