Fig. 4.

Activation of BMDCs in vitro following incubation. Cells isolated from C57BL6 mice were purified and cocultured with CpG-1826 and oxidized lysates (yellow bars), nonoxidized lysates (black bars), or no lysate (gray bars). After 2 d, DC activation was measured by flow cytometry for expression levels of (A) CD40, (B) CD80, (C) CD86, and (D) MHC-II. Statistical analysis was performed using an ordinary one-way ANOVA, where “*” represents a P value of <0.05, “**” represents a P value of <0.01, “***” represents a P value of <0.001, and “n.s.” represents a P value of >0.05, which is considered not significant. “MFI” denotes median fluorescence intensity.