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. 2020 Jul 14;117(30):17564–17570. doi: 10.1073/pnas.2006443117

Fig. 5.

Fig. 5.

MicroRNA detection based on the sharp transition from the classical to quantum coupling regimes. (A) Schematic illustration of microRNA detection using AuNPs coated with ssDNA. After hybridization with target microRNA and incubation with DSN, the ssDNA is cleaved, leading to a large reduction in the AuNP-Au film distance, and observed by the distinct switching in the phase of the SPR images. (B) SPR image of single AuNP. (C) Lateral displacement (x and y directions) of the AuNP shown in B before (blue dots) and after (black dots) adding DSN. (D) Line profile of AuNPs over time showing the cleavage process of the target microRNA:ssDNA hybridization. (E) Line profile of AuNPs over time for single-base mismatch microRNA:ssDNA hybridization incubating with DSN. (F) Distribution of DSN clearage time for different AuNPs. AuNP diameter: 100 nm.