Fig. 3.

BCA treatment ameliorated the TGF-β-induced expression of ECM markers: Cells were pre-treated with BCA for 2 h, thereafter rTGF-β added to the cells and cultured for 72 h. Further, cells were subjected to RT-qPCR for the specified transcripts in A-D) NHLF, E-H) DHLF, I-L) LL29.  Parallel dishes were subjected to immunofluorescence analysis for α-SMA protein. M-O) Immunofluorescence analysis in NHLF, DHLF and LL29 cells, respectively. Results were shown as Mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.