Fig. 5.

Role of BCA on TGF-β-induced fibrotic genes/markers expression in PCMLS:

PCMLS were pre-treated with BCA for 2 h, thereafter rTGF-β (mouse) added to the cells and cultured for 72 h. Further, PCMLS were subjected to RT-qPCR for the specified transcripts. A-G) Gene expression analysis.  Graphs in panels represent fold change in gene expression normalized to GAPDH. PCMLS derived cells were subjected to immunofluorescence analysis for H) FN-1, I) COL1A1 and J) E-cad. Results were shown as Mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.