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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Invest Dermatol. 2019 Nov 6;140(5):1035–1044.e7. doi: 10.1016/j.jid.2019.09.022

Figure 5. S7 treatment corrected abnormal GJ and hemichannel functions in KID-KCs.

Figure 5.

(a, b) SLDT was performed in siRNA-treated KID-KCs (KID) and control-KCs (Cont) to assess GJ activity, (c, d) Analysis of NB transfer (red). Each dot in panels (c) and (d) represents the average NB transfer from a single image. Three independent experiments were carried out and at least 10 images were analyzed from each experiment. Restoration of GJ activity was detected in KID-KCs following S7 treatment. Whole-cell patch clamp and NB uptake were carried out to examine hemichannel activity. (e–g) Records of currents from single cells under the voltage step protocol. (h) The plot of current density against membrane voltage shows correction of hyperactive hemichannels in KID-KCs after S7 treatment. (i) Representative NB (red) uptake images. The nuclei were stained with DAPI (blue). (j) Data analysis shows reversal of aberrantly enhanced NB uptake in KID-KCs. Data are presented as the mean ± SEM. Bar = 200 μm. *P < 0.05; **P < 0.01; ***P < 0.001. Control-KC, control keratinocyte; KID-KC, keratitis-ichthyosis-deafness syndrome-derived keratinocyte; NB, neurobiotin; N.S., not significant; SEM, standard error of the mean; si-cont, control small interfering RNA; SLDT, scrape-loading dye transfer.