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. 2020 Jul 28;13:7343–7352. doi: 10.2147/OTT.S240991

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© 2020 Chen et al.

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CXXC5 inhibited the activation of Wnt/β-catenin/Axin2 signaling in GC.

Notes: Cells were transfected by 0.5 μg pcDNA-CXXC5 with or without SKL2001 treatment before detections. (A and B) Using Western blotting method, the activation of Wnt/β-catenin/Axin2 pathway and expressions of EMT proteins were measured. (C and D) The migration and invasion of MKN-45 and AGS were detected by Transwell analysis. (E) The results of Flow cytometry presented apoptosis rate. *P < 0.05 and **P < 0.01 versus NC; ^#P < 0.05 versus CXXC5 group.