Figure 3.

miR-330-3p was the target of LINC01094 in gliomas. (A) LINC01094-WT and LINC01094-MUT luciferase reporter plasmids containing the binding site for miR-330-3p were constructed. (B) Dual luciferase reporter assay was used to detect the targeting relationship between miR-330-3p and LINC01094 in LN229 and U251 cells. (C and D) RNA pull-down assay in LN229 cells and RIP assay in U251 cells confirmed that LINC01094 and miR-330-3p were directly interacted. (E) qRT-PCR was used to detect the expression of miR-330-3p in LN229 cells with LINC01094 overexpression and U251 cells with LINC01094 knockdown. (F) qRT-PCR was used to detect the expression of LINC01094 in U251 cells with miR-330-3p overexpression and LN229 cells with miR-330-3p knockdown. Data were represented as mean±SD of at least three independent experiments. ***P<0.001.

Abbreviations: WT, wild type; MUT, mutant type; qRT-PCR, quantitative reverse transcription-PCR; RIP, RNA immunoprecipitation.