Figure 5.

MSI1 was a downstream target of miR-330-3p in gliomas. (A) MSI1-WT and MSI1-MUT luciferase reporter plasmids containing the binding site for miR-330-3p were constructed. (B) Dual luciferase reporter assay was used to detect the targeting relationship between miR-330-3p and MSI1 3ʹUTR in U251 and LN229 cells. (C) qRT-PCR was used to detect the mRNA expression of MSI1 in U251 cells transfected with miR-330-3p overexpression and LN229 cells transfected with miR-330-3p knockdown. (D) Western blot was used to detect MSI1 protein expression in U251 cells transfected with miR-330-3p overexpression and LN229 cells transfected with miR-330-3p knockdown. Data were represented as mean±SD of at least three independent experiments. **P<0.01, ***P<0.001.

Abbreviations: WT, wild type; MUT, mutant type; UTR, unsaturated region; qRT-PCR, quantitative reverse transcription-PCR.