Figure 6.

miR-330-3p partially reversed the tumor-promoting effect of LINC01094 on glioma cells. (A) Western blot was used to detect the relative expression of MSI1 in LN229 cells transfected with MSI1 plasmid and U251 cells transfected with a siRNA targeting MSI1. (B) CCK-8 was used to detect the proliferation of LN229 cells transfected with MSI1 or NC and U251 cells transfected with si-MSI1 or si-NC. (C) Transwell assay was used to detect the migration and invasion of LN229 cells transfected with MSI1 or NC and U251 cells transfected with si-MSI1 or si-NC. (D) miR-330-3p mimics were transfected into LN229 cells with LNC229094 overexpression, and anti-miR-330-3p was transfected into U251 cells with LINC01094 knockdown. 48 hours after transfection, qRT-PCR was used to detect the expression of miR-330-3p into LN229 and U251 cells. (E) Western blot was used to detect the expression of MSI1 protein in LN229 and U251 cells. (F) CCK-8 was used to detect the proliferation of LN229 and U251 cells. (G) Transwell assay was used to detect the migration and invasion of LN229 and U251 cells. Scale bar=20 µm. Data were represented as mean±SD of at least three independent experiments. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: qRT-PCR, quantitative reverse transcription-PCR; CCK-8, cell-counting kit-8.