Fig. 3.
RNF170 inhibits TLR3-triggered innate immune responses in vivo. a Sex- and age-matched Rnf170+/+ and Rnf170−/− mice (n = 4 per genotype) were injected intraperitoneally (i.p.) with EMCV (1 × 105 pfu per mouse) and sacrificed after 48 h. Ifna4, Ifnb1, Il6, and Tnf mRNA expression in the heart and brain was measured by qRT-PCR analysis. b EMCV titers in the heart and brain of the mice described in a were determined by a TCID50 assay. c Hematoxylin and eosin staining of heart sections from the mice described in a was shown. Scale bar, 50 μm. d Sex- and age-matched Rnf170+/+ (n = 11) and Rnf170−/− mice (n = 9) were injected intraperitoneally (i.p.) with EMCV (1 × 105 pfu per mouse). The survival rate of the injected mice was monitored every 24 h. Data are representative of three independent experiments (a–d). Data are shown as the mean ± SEM in a and b. *P < 0.05 and **P < 0.01; paired Student’s t-test