Table 2.

Definition of each term in Eq. (2) for both the behavioural and environmental rules.

Term	Behavioural rule	Environmental rule
A	ID of the cell type for which that reaction is available	ID of the cell type for which that reaction is available
B	ID of the cell type resulting from the execution of that rule	Result of the interaction, i.e. amount of nutrients/oxygen consumed, change in stiffness
C	Optional. Additional ID of the cell type resulting from the execution of that rule. Used to model cell doubling and migration	Not present
D	Probability of the reaction. This can be a function of any combination of the variables reported in Table 3, making it possible to simulate complex dynamics and describe realistic cell behaviours	Tag identifying the specific interaction modeled. “environment (Glc)”, “environment (O2)” and “environment (YM)” are currently available. Use the variable “U” to set glucose and oxygen consumption to reference values (glucose uptake: 16.7 10−12 g/cell/h49, oxygen uptake: 2.93 10−15 g/cell/h50