Fig. 2.

KDM2B deficiency decreases IL-6 production in response to LPS or poly(I:C) in innate immune cells. a Heat map of mRNA expression in Kdm2b^−/− (KO) and Kdm2b^+/+ (WT) peritoneal macrophages 3 h after LPS stimulation. b, c Q-PCR analysis of Il6 mRNA expression or ELISA of IL-6 production in supernatants of Kdm2b^−/− and Kdm2b^+/+ peritoneal macrophages (b) or mouse peritoneal macrophages transfected with control siRNA (Ctrl siRNA-1) or Kdm2b-specific siRNA (Kdm2b siRNA-1) (c) followed by stimulation with LPS or poly(I:C) for the indicated times. d, e Q-PCR analysis of Il6 mRNA expression or ELISA of IL-6 production in supernatants of Kdm2b^−/− and Kdm2b^+/+ BMDMs (d) and BMDCs (e) stimulated with LPS or poly(I:C) for 4 h. The data are from three independent experiments (b–e, mean ± s.e.m.). *P < 0.05; **P < 0.01