Fig. 3.

KDM2B selectively binds the Il6 promoter but does not affect its methylation modification. a, b ChIP followed by quantitative PCR (ChIP-qPCR) analysis of KDM2B occupancy at the Il6, Tnf and Ifnb1 promoters in Kdm2b^−/− (KO) and Kdm2b^+/+ (WT) peritoneal macrophages stimulated with LPS (a) or poly(I:C) (b) for the indicated times. c ChIP-qPCR analysis of H3K4me3 modification at the Il6 promoter in Kdm2b^−/− (KO) and Kdm2b^+/+ (WT) peritoneal macrophages stimulated with LPS or poly(I:C) for 2 h or left untreated. d Luciferase activity assay of Il6 promoter reporter activated by MyD88 in lysates of HEK 293T cells transfected with mock vector, wild-type KDM2B or a KDM2B mutant with a Jmjc domain deletion (KDM2B ΔJmjc) as indicated. The data are from three independent experiments (a–d, mean ± s.e.m.). **P < 0.01