Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 31;11:3847. doi: 10.1038/s41467-020-17497-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Confocal imaging of N. benthamiana protoplasts expressing three 3WJ-4 × Bro-tagged mRNAs. Scale bars, 25 μm. b Signal-to-background ratio of fluorescence in protoplasts incubated with 10 μM DFHBI-1T. Error bars, mean ± s.d. (n = 18). c Representative confocal images of N. benthamiana leaves expressing three 3WJ-4 × Bro-tagged mRNAs. Scale bars, 25 μm. d Detection of fluorescence and background in leaf cells. The blue lines in c were used for the line scan in d. e Simultaneous detection of mCherry-3WJ-4 × Bro mRNA and mCherry protein in N. benthamiana leaves by confocal laser microscopy. RNA of mCherry-3WJ-4 × Bro was imaged at 488-nm excitation and 527-nm emission and mCherry protein at 560-nm excitation and 610-nm emission. Scale bars, 75 μm. f Confocal microscopy detection of dynamic nuclear export process of NtTubα mRNA with 3WJ-4 × Bro in N. benthamiana leaf cells at 0, 24, 48, and 72-h post infiltration, respectively. Scale bars, 75 μm. All experiments were repeated three independent times with the same conclusion. Source Data are provided as a Source Data file.