Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 31;11:3847. doi: 10.1038/s41467-020-17497-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Schematic diagram of the Agrobacterium binary vector system containing the TRV RNA1 and RNA2 genomes with some modification, as well as the experimental scheme of the TRV-mediated target RNA delivery. RNA1 contains LB (left border), 2 × 35S (2 × CaMV 35S promoter), RdRNAP (encoding RNA-dependent RNA polymerase), MP (encoding movement protein), 16k (encoding a cysteine-rich protein), Rz (self-cleaving ribozyme), Tnos (nopaline synthase terminator), and RB (right border). RNA2 contains LB, p35S, CP (encoding coat protein), PEBV (pea early browning virus promoter), Rz, Tnos, and RB. mCherry-3WJ-4 × Bro was cloned under the control of PEBV. The flask, tube, and syringe are drawn by software Photoshop CS6. b Fluorescence signals of N. benthamiana leaves under ultraviolet and confocal microscopes. Agrobacterium cultures carrying the engineered TRV RNA2 and RNA2 vectors were co-infiltrated into N. benthamiana leaves to express and transmit 3WJ-4 × Bro-tagged RNA (mCherry-3WJ-4 × Bro). At 5 days after Agrobacterium infection, both infiltrated and systemic leaves were sampled and then incubated with 10-μM DFHBI-1T at room temperature for 20 min. Scale bars, 75 μm. Three independent trials showed the same results. c Confirmation by RT-PCR of the presence of the fusion RNA (mCherry-3WJ-4 × Bro) in both infiltrated and systemic leaves. Source Data underlying b are provided as a Source Data file.