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. 2020 Jul 31;12:22. doi: 10.1038/s41368-020-00088-z

Fig. 4.

Fig. 4

Analysis of the mRNA and protein expression of osteogenic differentiation markers during the osteogenic differentiation of DFCs. a Histograms showing the relative mRNA levels of ALP, BSP, OCN, and RUNX2 during the induction of differentiation. The mRNA levels were determined using RT-PCR with GAPDH as a reference for normalization. b Representative western blots showing the protein levels of ALP, BSP, OCN, and RUNX2. c Histograms showing the relative protein levels of ALP, BSP, OCN, and RUNX2 during the induction of differentiation. Values are means ± SD of three independent experiments (*P < 0.05 and **P < 0.01)