Fig. 2.

Lycorine blocked HMGB1/TLRs/NF-κB pathway in LPS-treated mice. One hour before challenged with LPS (2 mg/kg, dissolved in 50 μl saline) for 12 h, BALB/c mice were treated with or without lycorine (20 mg/kg or 40 mg/kg). 1, control group; 2, LPS group; 3, LPS + lycorine (20 mg/kg) group; 4, LPS + lycorine (40 mg/kg) group. a The expression of HMGB1 in the lung tissues was measured by immunohistochemistry assay, Scale bar = 100 μm. b Relative protein expression levels of HMGB1, TLR4, TLR5, Myc88, P65, P-P65, IκBα, and P-IκBα in the lung tissues were determined by Western blot analysis. β-actin as an internal control. c The mRNA expression levels of HMGB1, TLR4, and TLR5 in each group were measured by RT-qPCR. N = 6. Data are means ± SD. A superscript with the same letter (such as a and ab) means that the difference is not significant, and a superscript with different letter (such as a and b) means that the difference is significant (P < 0.05). LPS lipopolysaccharide, HMGB1 the high-mobility group box 1, TLR Toll-like receptor, RT-qPCR quantitative real-time PCR