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. 2020 Jul 31;10:12978. doi: 10.1038/s41598-020-69928-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Example of spatiotemporal maps generated through the ImageJ software (version 1.51q; NIH, Bethesda, MD, USA, 2017) with the DMapLE plug-in (developed by Dr. Sean Parsons and freely available at https://scepticalphysiologist.com/code/code.html) for recordings of cecal contractions in laying hens. Maps display the diameter across the cecum from the cranial to caudal end vertically (cm) and over time (s) horizontally. The diameter of the gut is displayed as bands of light (relaxation; larger diameter) and dark (contraction; smaller diameter) hues (a). In the current example, four contractions running from the cranial to the caudal end can be observed (indicated with arrows). Velocity is determined by measuring the slope of contraction, frequency as the number of contractions within a given time period, and amplitude as the height of the diameter change at peak contraction (a). Note that these are examples and therefore the bands in a do not correspond to the peaks in (b).