Table 2.

Precision and accuracy for HPLC-MS/MS analysis of 6-O-demethylmenisporphine in rat plasma, liver, urine, bile and feces (n = 6).

Biosamples	QC concentration (ng/mL)	Intra-day		Inter-day
		Precision (RSD, %)	Accuracy (RE, %)	Precision (RSD, %)	Accuracy (RE, %)
Plasma	5	8.2	−8.5	9.5	−5.6
	15	6.2	−2.0	9.1	−2.6
	150	7.1	−3.8	8.4	−9.1
	1600	3.6	−2.8	10.1	−7.9
Liver	2	3.5	−5.4	7.2	−6.4
	6	6.3	10.7	8.2	5.4
	80	3.5	−10.2	5.4	−8.0
	800	5.4	5.5	8.4	−10.9
Urine	2	7.9	−8.2	8.3	−1.5
	6	10.0	7.7	9.8	8.9
	80	6.7	−6.1	7.0	−5.0
	800	5.4	−6.5	5.9	−7.0
Bile	2	9.0	−4.3	8.5	5.7
	6	8.7	−1.9	11.0	4.1
	80	5.4	−3.2	6.2	−7.0
	800	6.8	5.4	7.6	11.2
Feces	2	7.6	8.9	9.7	−5.5
	6	11.1	−10.9	9.0	−10.5
	80	9.5	9.8	10.3	12.2
	800	7.7	8.6	9.0	13.0