Table 3.
Extraction recovery and matrix effect of 6-O-demethylmenisporphine and IS in rat plasma, liver, urine, bile and feces (n = 6).
| Sample matrix | Analytes | QC concentration (ng/mL) | Recovery (%) | RSD (%) | Matrix effect (%) | RSD (%) |
|---|---|---|---|---|---|---|
| Plasma | 6-O-demethylmenisporphine | 15 | 88.4 ± 7.4 | 8.4 | 110.9 ± 3.6 | 3.2 |
| 150 | 85.5 ± 6.2 | 7.3 | 102.4 ± 5.5 | 5.4 | ||
| 1600 | 93.2 ± 6.1 | 6.5 | 109.3 ± 2.5 | 2.3 | ||
| IS | 40 | 80.1 ± 4.0 | 5.0 | 90.2 ± 3.8 | 4.2 | |
| Liver | 6-O-demethylmenisporphine | 6 | 91.5 ± 7.9 | 8.6 | 100.9 ± 12.7 | 12.6 |
| 80 | 88.2 ± 7.2 | 8.2 | 101.2 ± 6.7 | 6.6 | ||
| 800 | 92.4 ± 8.5 | 9.2 | 105.3 ± 6.7 | 6.4 | ||
| IS | 40 | 88.2 ± 7.9 | 9.0 | 106.2 ± 12.1 | 11.4 | |
| Urine | 6-O-demethylmenisporphine | 6 | 91.3 ± 12.0 | 13.1 | 105.5 ± 8.4 | 7.9 |
| 80 | 94.2 ± 5.2 | 5.5 | 98.3 ± 5.9 | 6.0 | ||
| 800 | 90.1 ± 6.8 | 7.5 | 95.7 ± 7.0 | 7.3 | ||
| IS | 40 | 90.9 ± 5.4 | 5.9 | 86.1 ± 6.3 | 7.3 | |
| Bile | 6-O-demethylmenisporphine | 6 | 97.6 ± 13.3 | 13.6 | 89.0 ± 12.6 | 14.1 |
| 80 | 85.9 ± 9.7 | 11.3 | 86.7 ± 11.3 | 13.0 | ||
| 800 | 89.9 ± 7.9 | 8.8 | 88.4 ± 9.0 | 10.2 | ||
| IS | 40 | 81.6 ± 11.7 | 14.4 | 85.9 ± 6.9 | 8.0 | |
| Feces | 6-O-demethylmenisporphine | 6 | 101.3 ± 9.7 | 9.5 | 90.3 ± 10.5 | 11.7 |
| 80 | 94.3 ± 8.9 | 9.4 | 93.1 ± 11.2 | 12.0 | ||
| 800 | 84.5 ± 3.8 | 4.5 | 87.8 ± 10.0 | 11.4 | ||
| IS | 40 | 80.2 ± 8.9 | 11.1 | 87.1 ± 11.2 | 12.9 |