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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Stem Cell Rev Rep. 2020 Aug;16(4):684–692. doi: 10.1007/s12015-020-09974-9

Figure 2: Old MSCs can induce HSPCs aging phenotype.

Figure 2:

(a) Schematic representation of culture system young HSPC with ex vivo expanded MSCs from young or old mice. (b) Quantitation of total hematopoietic cells and LSK expansion after culture of young HSPC with MSC from young or old mice for 7 days. Data are X±SEM; N= 5 independent cocultures; *=P<0.05. (c-d) Measurement of repopulation and trilineage differentiation capacity of HSPC co-cultured on young or old MSC in a competitive transplantation model. HSPC cultured on young or old MSC were mixed with competitor cells and transplanted into lethally irradiated young mice. At 6-month post transplantation, donor cell engraftment and myeloid and lymphoid frequency in the PB of recipient mice was measured by flow cytometry. Data are X±SEM; N= 4 mice/group, each assayed individually; *=P<0.05.