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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Stem Cell Rev Rep. 2020 Aug;16(4):684–692. doi: 10.1007/s12015-020-09974-9

Figure 4: CXCR4 deficiency in MSC induces HSPC aging phenotype.

Figure 4:

(a) Schematic of WT HSPC co-cultured with ex vivo expanded MSC from WT or CXCR4 KO mice. (b) Measurement of CXCR4 expression on BM MSC of tamoxifen treated WT and CXCR4 conditional KO mice by flow cytometry. (c-d) LSK frequency and cycling after culturing the WT HSPC for 7 days on WT or CXCR4 KO MSC. (e-f) Measurement of hematopoietic reconstitution and myeloid differentiation capacity of WT HSPC cultured on WT or CXCR4 KO MSC after competitive transplantation. HSPC cultured on WT or CXCR4 KO MSC were mixed with competitor cells and transplanted into lethally irradiated young mice. At 6-month post transplantation, donor cell chimerism and myeloid cell and lymphoid frequency in the BM of recipient mice was measured by flow cytometry. Data are X±SEM; N=4–5 mice/group, each assayed individually; *=P<0.05.