Table 1.

Limits of detection and quantification of the 1:7 dilution protocol in HIV-1 RNA controls

	APT-Quant-dil
Reference Value	mean	SD	Quantified	Detected
(log10 cop/mL)	(log10 cop/mL)		(%)	(%)
1.56	0	0	0	80
1.89	0	0	0	93
2.33	2.43	0.1	33	100
2.49	2.50	0.12	60	100
2.56	2.51	0.12	67	100
2.62	2.61	0.11	93	100
2.76	2.75	0.18	100	100
2.93	2.89	0.12	100	100

Quantified results from diluted HIV-1 RNA controls tested in triplicate with APT-Quant-std to determine the reference value and tested in 15 replicates with APT-Quant-dil to determine percent quantified and detected, and the mean and standard deviation (SD).