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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Am J Transplant. 2020 Mar 24;20(8):2206–2215. doi: 10.1111/ajt.15837

Figure 2: FcγRIIB functions intrinsically to inhibit CD8+ T cell responses to Listeria.

Figure 2:

WT OVA-specific CD45.2+ CD8+ T cells (WT OT-I) or Fcgr2b−/− OVA-specific CD45.2+ CD8+ T cells (Fcgr2b−/− OT-I) were harvested from the spleen and adoptively transferred into WT CD45.1+ C57BL/6 hosts that were then infected with Listeria and bled on days 4, 7, 10, 14, and 21. A) Schematic of experimental design. B-C) Representative flow plots and summary data of the frequency of FcγRIIB-expressing naïve and WT OT-I T cells on days 7, 10, and 14. Due to low cell number, FcγRIIB frequency could not be assessed on day 21. Representative data from two independent experiments, n=5 mice per group. D) Representative flow plots of the frequency of WT and Fcgr2b−/− OT-I T cells on days 7, 10, and 14. E) Summary data of the frequency of WT and FcγRIIB−/− OT-I T cells in the blood on days 4, 7, 10, 14 and 21. Representative data from two independent experiments, n=5 mice per group. Two-way ANOVA was performed, **p<0.01. F-G) Summary data of the frequency and absolute numbers of WT and Fcgr2b−/− OT-I T cells in the blood on days 10 and 14. Pooled data from 2–3 independent experiments, n=5 mice per group. Mann-Whitney t test was performed, **p<0.01.